Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD
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Matić, Gordana
Vojnović-Milutinović, Danijela

Nestorov, Jelena

Elaković, Ivana
Manitašević-Jovanović, Sanja

Perišić, Tatjana
Dunderski, Jadranka
Damjanović, Svetozar
Knežević, Goran

Spirić, Željko
Vermetten, Eric

Savić, Danka

Article (Published version)

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Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the recept...or was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs.
Keywords:
War trauma / Resilience to PTSD / PTSD / Peripheral blood mononuclear cells / Glucocorticoid receptorSource:
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 2013, 43, 238-245Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
- European Commission Joint Research Centre [INCO-CT-2004-509213]
- Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (RS-41009)
- Identification, measurement and development of the cognitive and emotional competences important for a Europe-oriented society (RS-179018)
DOI: 10.1016/j.pnpbp.2013.01.005
ISSN: 0278-5846
PubMed: 23333536
WoS: 000317878700031
Scopus: 2-s2.0-84873550690
Institution/Community
Psihologija / PsychologyTY - JOUR AU - Matić, Gordana AU - Vojnović-Milutinović, Danijela AU - Nestorov, Jelena AU - Elaković, Ivana AU - Manitašević-Jovanović, Sanja AU - Perišić, Tatjana AU - Dunderski, Jadranka AU - Damjanović, Svetozar AU - Knežević, Goran AU - Spirić, Željko AU - Vermetten, Eric AU - Savić, Danka PY - 2013 UR - http://reff.f.bg.ac.rs/handle/123456789/1626 AB - Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Progress in Neuro-Psychopharmacology & Biological Psychiatry T1 - Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD EP - 245 SP - 238 VL - 43 DO - 10.1016/j.pnpbp.2013.01.005 ER -
@article{ author = "Matić, Gordana and Vojnović-Milutinović, Danijela and Nestorov, Jelena and Elaković, Ivana and Manitašević-Jovanović, Sanja and Perišić, Tatjana and Dunderski, Jadranka and Damjanović, Svetozar and Knežević, Goran and Spirić, Željko and Vermetten, Eric and Savić, Danka", year = "2013", abstract = "Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Progress in Neuro-Psychopharmacology & Biological Psychiatry", title = "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD", pages = "245-238", volume = "43", doi = "10.1016/j.pnpbp.2013.01.005" }
Matić, G., Vojnović-Milutinović, D., Nestorov, J., Elaković, I., Manitašević-Jovanović, S., Perišić, T., Dunderski, J., Damjanović, S., Knežević, G., Spirić, Ž., Vermetten, E.,& Savić, D.. (2013). Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-Psychopharmacology & Biological Psychiatry Pergamon-Elsevier Science Ltd, Oxford., 43, 238-245. https://doi.org/10.1016/j.pnpbp.2013.01.005
Matić G, Vojnović-Milutinović D, Nestorov J, Elaković I, Manitašević-Jovanović S, Perišić T, Dunderski J, Damjanović S, Knežević G, Spirić Ž, Vermetten E, Savić D. Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2013;43:238-245. doi:10.1016/j.pnpbp.2013.01.005 .
Matić, Gordana, Vojnović-Milutinović, Danijela, Nestorov, Jelena, Elaković, Ivana, Manitašević-Jovanović, Sanja, Perišić, Tatjana, Dunderski, Jadranka, Damjanović, Svetozar, Knežević, Goran, Spirić, Željko, Vermetten, Eric, Savić, Danka, "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD" in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 43 (2013):238-245, https://doi.org/10.1016/j.pnpbp.2013.01.005 . .