REFF - Faculty of Philosophy Repository
University of Belgrade - Faculty of Philosophy
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   REFF
  • Psihologija / Psychology
  • Radovi istraživača / Researcher's publications - Odeljenje za psihologiju
  • View Item
  •   REFF
  • Psihologija / Psychology
  • Radovi istraživača / Researcher's publications - Odeljenje za psihologiju
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD

No Thumbnail
Authors
Matić, Gordana
Vojnović-Milutinović, Danijela
Nestorov, Jelena
Elaković, Ivana
Manitašević-Jovanović, Sanja
Perišić, Tatjana
Dunderski, Jadranka
Damjanović, Svetozar
Knežević, Goran
Spirić, Željko
Vermetten, Eric
Savić, Danka
Article (Published version)
Metadata
Show full item record
Abstract
Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the recept...or was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs.

Keywords:
War trauma / Resilience to PTSD / PTSD / Peripheral blood mononuclear cells / Glucocorticoid receptor
Source:
Progress in Neuro-Psychopharmacology & Biological Psychiatry, 2013, 43, 238-245
Publisher:
  • Pergamon-Elsevier Science Ltd, Oxford
Funding / projects:
  • European Commission Joint Research Centre [INCO-CT-2004-509213]
  • Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (RS-41009)
  • Identification, measurement and development of the cognitive and emotional competences important for a Europe-oriented society (RS-179018)

DOI: 10.1016/j.pnpbp.2013.01.005

ISSN: 0278-5846

PubMed: 23333536

WoS: 000317878700031

Scopus: 2-s2.0-84873550690
[ Google Scholar ]
33
35
URI
http://reff.f.bg.ac.rs/handle/123456789/1626
Collections
  • Radovi istraživača / Researcher's publications - Odeljenje za psihologiju
Institution/Community
Psihologija / Psychology
TY  - JOUR
AU  - Matić, Gordana
AU  - Vojnović-Milutinović, Danijela
AU  - Nestorov, Jelena
AU  - Elaković, Ivana
AU  - Manitašević-Jovanović, Sanja
AU  - Perišić, Tatjana
AU  - Dunderski, Jadranka
AU  - Damjanović, Svetozar
AU  - Knežević, Goran
AU  - Spirić, Željko
AU  - Vermetten, Eric
AU  - Savić, Danka
PY  - 2013
UR  - http://reff.f.bg.ac.rs/handle/123456789/1626
AB  - Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Progress in Neuro-Psychopharmacology & Biological Psychiatry
T1  - Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD
EP  - 245
SP  - 238
VL  - 43
DO  - 10.1016/j.pnpbp.2013.01.005
ER  - 
@article{
author = "Matić, Gordana and Vojnović-Milutinović, Danijela and Nestorov, Jelena and Elaković, Ivana and Manitašević-Jovanović, Sanja and Perišić, Tatjana and Dunderski, Jadranka and Damjanović, Svetozar and Knežević, Goran and Spirić, Željko and Vermetten, Eric and Savić, Danka",
year = "2013",
abstract = "Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Progress in Neuro-Psychopharmacology & Biological Psychiatry",
title = "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD",
pages = "245-238",
volume = "43",
doi = "10.1016/j.pnpbp.2013.01.005"
}
Matić, G., Vojnović-Milutinović, D., Nestorov, J., Elaković, I., Manitašević-Jovanović, S., Perišić, T., Dunderski, J., Damjanović, S., Knežević, G., Spirić, Ž., Vermetten, E.,& Savić, D.. (2013). Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-Psychopharmacology & Biological Psychiatry
Pergamon-Elsevier Science Ltd, Oxford., 43, 238-245.
https://doi.org/10.1016/j.pnpbp.2013.01.005
Matić G, Vojnović-Milutinović D, Nestorov J, Elaković I, Manitašević-Jovanović S, Perišić T, Dunderski J, Damjanović S, Knežević G, Spirić Ž, Vermetten E, Savić D. Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2013;43:238-245.
doi:10.1016/j.pnpbp.2013.01.005 .
Matić, Gordana, Vojnović-Milutinović, Danijela, Nestorov, Jelena, Elaković, Ivana, Manitašević-Jovanović, Sanja, Perišić, Tatjana, Dunderski, Jadranka, Damjanović, Svetozar, Knežević, Goran, Spirić, Željko, Vermetten, Eric, Savić, Danka, "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD" in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 43 (2013):238-245,
https://doi.org/10.1016/j.pnpbp.2013.01.005 . .

DSpace software copyright © 2002-2015  DuraSpace
About REFF | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About REFF | Send Feedback

OpenAIRERCUB