Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis
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2021
Authors
Petrović, BojanPantelinac, Jelena
Capo, Ivan
Miljković, Dejan
Popović, Milan
Penezić, Kristina
Stefanović, Sofija

Article (Published version)

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The aim of the present study was to evaluate the effect of different staining techniques on applicability and accuracy of tooth cement tun annulation (TCA) method. Nine decalcination techniques, 8 dehydration protocols and 8 different techniques were applied in 3 teeth from the persons of a known age. Black and white, and color images of histological sections were captured. An x-ray was taken of each tooth and they were photographed. Researchers were asked to observe both black/white and color images of histological sections. Researchers were divided into two groups. The first group analyzed histological images only, and the second group had photos of teeth and X-rays. In the first group of observers (without X ray) the differences in age estimation between real and observed age were significant for 2 younger patients, but not for the oldest patient, where the observed and real values matched. Of the 6 raters, the assesments of the last 3 (that used x-ray images together with histologi...cal sections) did not differ significantly from the real values. Extensive analysis and multiple repetitions performed in the present investigation revealed that the most optimal method of decalcification for TCA method was EDTA II for a period longer than 14 days at a section thickness of 2-3nun, while the most optimal protocol for dehydration was number IV. When it comes to staining, the most optimal staining protocol used for the cemental lines visualization and counting was Crocein Scarlet/Acid Fuchsin staining and Toluidine blue staining used at semithin section. Additional use of preexperimental evaluation employing x-ray of analyzed teeth decreased the errors of age estimation.
Keywords:
Increment lines / Dental cementum / Age estimationSource:
International Journal of Morphology, 2021, 39, 1, 216-221Publisher:
- Universidad de la Frontera
Funding / projects:
DOI: 10.4067/S0717-95022021000100216
ISSN: 0717-9367
WoS: 000609557500034
Scopus: 2-s2.0-85099762222
Institution/Community
Arheologija / ArchaeologyTY - JOUR AU - Petrović, Bojan AU - Pantelinac, Jelena AU - Capo, Ivan AU - Miljković, Dejan AU - Popović, Milan AU - Penezić, Kristina AU - Stefanović, Sofija PY - 2021 UR - http://reff.f.bg.ac.rs/handle/123456789/3289 AB - The aim of the present study was to evaluate the effect of different staining techniques on applicability and accuracy of tooth cement tun annulation (TCA) method. Nine decalcination techniques, 8 dehydration protocols and 8 different techniques were applied in 3 teeth from the persons of a known age. Black and white, and color images of histological sections were captured. An x-ray was taken of each tooth and they were photographed. Researchers were asked to observe both black/white and color images of histological sections. Researchers were divided into two groups. The first group analyzed histological images only, and the second group had photos of teeth and X-rays. In the first group of observers (without X ray) the differences in age estimation between real and observed age were significant for 2 younger patients, but not for the oldest patient, where the observed and real values matched. Of the 6 raters, the assesments of the last 3 (that used x-ray images together with histological sections) did not differ significantly from the real values. Extensive analysis and multiple repetitions performed in the present investigation revealed that the most optimal method of decalcification for TCA method was EDTA II for a period longer than 14 days at a section thickness of 2-3nun, while the most optimal protocol for dehydration was number IV. When it comes to staining, the most optimal staining protocol used for the cemental lines visualization and counting was Crocein Scarlet/Acid Fuchsin staining and Toluidine blue staining used at semithin section. Additional use of preexperimental evaluation employing x-ray of analyzed teeth decreased the errors of age estimation. PB - Universidad de la Frontera T2 - International Journal of Morphology T1 - Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis EP - 221 IS - 1 SP - 216 VL - 39 DO - 10.4067/S0717-95022021000100216 ER -
@article{ author = "Petrović, Bojan and Pantelinac, Jelena and Capo, Ivan and Miljković, Dejan and Popović, Milan and Penezić, Kristina and Stefanović, Sofija", year = "2021", abstract = "The aim of the present study was to evaluate the effect of different staining techniques on applicability and accuracy of tooth cement tun annulation (TCA) method. Nine decalcination techniques, 8 dehydration protocols and 8 different techniques were applied in 3 teeth from the persons of a known age. Black and white, and color images of histological sections were captured. An x-ray was taken of each tooth and they were photographed. Researchers were asked to observe both black/white and color images of histological sections. Researchers were divided into two groups. The first group analyzed histological images only, and the second group had photos of teeth and X-rays. In the first group of observers (without X ray) the differences in age estimation between real and observed age were significant for 2 younger patients, but not for the oldest patient, where the observed and real values matched. Of the 6 raters, the assesments of the last 3 (that used x-ray images together with histological sections) did not differ significantly from the real values. Extensive analysis and multiple repetitions performed in the present investigation revealed that the most optimal method of decalcification for TCA method was EDTA II for a period longer than 14 days at a section thickness of 2-3nun, while the most optimal protocol for dehydration was number IV. When it comes to staining, the most optimal staining protocol used for the cemental lines visualization and counting was Crocein Scarlet/Acid Fuchsin staining and Toluidine blue staining used at semithin section. Additional use of preexperimental evaluation employing x-ray of analyzed teeth decreased the errors of age estimation.", publisher = "Universidad de la Frontera", journal = "International Journal of Morphology", title = "Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis", pages = "221-216", number = "1", volume = "39", doi = "10.4067/S0717-95022021000100216" }
Petrović, B., Pantelinac, J., Capo, I., Miljković, D., Popović, M., Penezić, K.,& Stefanović, S.. (2021). Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis. in International Journal of Morphology Universidad de la Frontera., 39(1), 216-221. https://doi.org/10.4067/S0717-95022021000100216
Petrović B, Pantelinac J, Capo I, Miljković D, Popović M, Penezić K, Stefanović S. Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis. in International Journal of Morphology. 2021;39(1):216-221. doi:10.4067/S0717-95022021000100216 .
Petrović, Bojan, Pantelinac, Jelena, Capo, Ivan, Miljković, Dejan, Popović, Milan, Penezić, Kristina, Stefanović, Sofija, "Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis" in International Journal of Morphology, 39, no. 1 (2021):216-221, https://doi.org/10.4067/S0717-95022021000100216 . .