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Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis

Authorized Users Only
2021
Authors
Petrović, Bojan
Pantelinac, Jelena
Capo, Ivan
Miljković, Dejan
Popović, Milan
Penezić, Kristina
Stefanović, Sofija
Article (Published version)
Metadata
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Abstract
The aim of the present study was to evaluate the effect of different staining techniques on applicability and accuracy of tooth cement tun annulation (TCA) method. Nine decalcination techniques, 8 dehydration protocols and 8 different techniques were applied in 3 teeth from the persons of a known age. Black and white, and color images of histological sections were captured. An x-ray was taken of each tooth and they were photographed. Researchers were asked to observe both black/white and color images of histological sections. Researchers were divided into two groups. The first group analyzed histological images only, and the second group had photos of teeth and X-rays. In the first group of observers (without X ray) the differences in age estimation between real and observed age were significant for 2 younger patients, but not for the oldest patient, where the observed and real values matched. Of the 6 raters, the assesments of the last 3 (that used x-ray images together with histologi...cal sections) did not differ significantly from the real values. Extensive analysis and multiple repetitions performed in the present investigation revealed that the most optimal method of decalcification for TCA method was EDTA II for a period longer than 14 days at a section thickness of 2-3nun, while the most optimal protocol for dehydration was number IV. When it comes to staining, the most optimal staining protocol used for the cemental lines visualization and counting was Crocein Scarlet/Acid Fuchsin staining and Toluidine blue staining used at semithin section. Additional use of preexperimental evaluation employing x-ray of analyzed teeth decreased the errors of age estimation.

Keywords:
Increment lines / Dental cementum / Age estimation
Source:
International Journal of Morphology, 2021, 39, 1, 216-221
Publisher:
  • Universidad de la Frontera
Funding / projects:
  • Bioarchaeology of Ancient Europe: People, Animals and Plants in the Prehistory of Serbia (RS-47001)

DOI: 10.4067/S0717-95022021000100216

ISSN: 0717-9367

WoS: 000609557500034

Scopus: 2-s2.0-85099762222
[ Google Scholar ]
4
URI
http://reff.f.bg.ac.rs/handle/123456789/3289
Collections
  • Radovi istraživača / Researcher's publications - Odeljenje za arheologiju
Institution/Community
Arheologija / Archaeology
TY  - JOUR
AU  - Petrović, Bojan
AU  - Pantelinac, Jelena
AU  - Capo, Ivan
AU  - Miljković, Dejan
AU  - Popović, Milan
AU  - Penezić, Kristina
AU  - Stefanović, Sofija
PY  - 2021
UR  - http://reff.f.bg.ac.rs/handle/123456789/3289
AB  - The aim of the present study was to evaluate the effect of different staining techniques on applicability and accuracy of tooth cement tun annulation (TCA) method. Nine decalcination techniques, 8 dehydration protocols and 8 different techniques were applied in 3 teeth from the persons of a known age. Black and white, and color images of histological sections were captured. An x-ray was taken of each tooth and they were photographed. Researchers were asked to observe both black/white and color images of histological sections. Researchers were divided into two groups. The first group analyzed histological images only, and the second group had photos of teeth and X-rays. In the first group of observers (without X ray) the differences in age estimation between real and observed age were significant for 2 younger patients, but not for the oldest patient, where the observed and real values matched. Of the 6 raters, the assesments of the last 3 (that used x-ray images together with histological sections) did not differ significantly from the real values. Extensive analysis and multiple repetitions performed in the present investigation revealed that the most optimal method of decalcification for TCA method was EDTA II for a period longer than 14 days at a section thickness of 2-3nun, while the most optimal protocol for dehydration was number IV. When it comes to staining, the most optimal staining protocol used for the cemental lines visualization and counting was Crocein Scarlet/Acid Fuchsin staining and Toluidine blue staining used at semithin section. Additional use of preexperimental evaluation employing x-ray of analyzed teeth decreased the errors of age estimation.
PB  - Universidad de la Frontera
T2  - International Journal of Morphology
T1  - Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis
EP  - 221
IS  - 1
SP  - 216
VL  - 39
DO  - 10.4067/S0717-95022021000100216
ER  - 
@article{
author = "Petrović, Bojan and Pantelinac, Jelena and Capo, Ivan and Miljković, Dejan and Popović, Milan and Penezić, Kristina and Stefanović, Sofija",
year = "2021",
abstract = "The aim of the present study was to evaluate the effect of different staining techniques on applicability and accuracy of tooth cement tun annulation (TCA) method. Nine decalcination techniques, 8 dehydration protocols and 8 different techniques were applied in 3 teeth from the persons of a known age. Black and white, and color images of histological sections were captured. An x-ray was taken of each tooth and they were photographed. Researchers were asked to observe both black/white and color images of histological sections. Researchers were divided into two groups. The first group analyzed histological images only, and the second group had photos of teeth and X-rays. In the first group of observers (without X ray) the differences in age estimation between real and observed age were significant for 2 younger patients, but not for the oldest patient, where the observed and real values matched. Of the 6 raters, the assesments of the last 3 (that used x-ray images together with histological sections) did not differ significantly from the real values. Extensive analysis and multiple repetitions performed in the present investigation revealed that the most optimal method of decalcification for TCA method was EDTA II for a period longer than 14 days at a section thickness of 2-3nun, while the most optimal protocol for dehydration was number IV. When it comes to staining, the most optimal staining protocol used for the cemental lines visualization and counting was Crocein Scarlet/Acid Fuchsin staining and Toluidine blue staining used at semithin section. Additional use of preexperimental evaluation employing x-ray of analyzed teeth decreased the errors of age estimation.",
publisher = "Universidad de la Frontera",
journal = "International Journal of Morphology",
title = "Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis",
pages = "221-216",
number = "1",
volume = "39",
doi = "10.4067/S0717-95022021000100216"
}
Petrović, B., Pantelinac, J., Capo, I., Miljković, D., Popović, M., Penezić, K.,& Stefanović, S.. (2021). Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis. in International Journal of Morphology
Universidad de la Frontera., 39(1), 216-221.
https://doi.org/10.4067/S0717-95022021000100216
Petrović B, Pantelinac J, Capo I, Miljković D, Popović M, Penezić K, Stefanović S. Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis. in International Journal of Morphology. 2021;39(1):216-221.
doi:10.4067/S0717-95022021000100216 .
Petrović, Bojan, Pantelinac, Jelena, Capo, Ivan, Miljković, Dejan, Popović, Milan, Penezić, Kristina, Stefanović, Sofija, "Using Histological Staining Techniques to Improve Visualization and Interpretability of Tooth Cementum Annulation Analysis" in International Journal of Morphology, 39, no. 1 (2021):216-221,
https://doi.org/10.4067/S0717-95022021000100216 . .

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